Identification of Blood Protozoa Infestation Transmitted by Vector Tikes among Awassi Sheep Herds in Kifri City, Kurdistan Region of Iraq

Authors

  • Mahmood Ahmad Hossein Department of Animal Production, Collage of Agricultural Engineering Science, University of Garmian, Kalar, As-Sulaymaniyah, KRG, Iraq

DOI:

https://doi.org/10.21928/uhdjst.v7n2y2023.pp1-5

Keywords:

Babesia ovis, Babesia mutasi, Kifri, Rhipicephalus bursa , Sheep

Abstract

Blood protozoan disease is a common disease among animals in the Kifri city, Kurdistan region of Iraq that this disease is mostly transmitted by ticks. Therefore, the present study aimed to investigate the level of blood protozoan and to identify vector ticks in the native breed sheep (Awassi sheep) in Kifri city. For this purpose, blood samples were taken from 150 sheep suspected suffering from protozoan infection according to their clinical symptoms. In the present study, we prepared blood slides from suspected sheep and stained with Giemsa staining, and then at the same time, hard ticks were collected from the sheep’s body. Then, the protozoan type was diagnosed and the vector tick species were identified by microscopically. The obtained results were statistically analyzed by the chi-square test. The results showed that 35 (23.33%) of that samples were infected with Babesia protozoa as 25 samples (16.66%) were infected with Babesia ovis, seven samples (4.66%) with Babesia mutasi, and three samples (2%) with B. ovis and B. mutasi. No infestation with Theileria and Anaplasma species was found. Rhipicephalus, Hyalomma, Dermacentor, and Haemaphysalis ticks were isolated and identified from the studied sheep. The results showed that the presence of the Rhipicephalus bursa tick is significantly (P < 0.05) related to the existence of Babesiosis disease in sheep. This study concluded that most of the studied sheep in Kifri city are infected with Babesia protozoa, especially B. ovis.

References

FAO. “Quarterly Bulletin of Statistics”. Vol. 1. FAO, Rome, Italy, 2020, p. 234.

Ministry of Planning, “Means and prospects and Sheep and Goat development in Iraq”, 2022, p. 124.

Q. Liu, Y. Q. Zhou and D. N. Zhou. “Semi-nested PCR detection of Babesia orientalis in its natural hosts Rhipicephalus haemaphysaloides and buffalo”. Veterinary Parasitology, vol. 143, pp. 260-266, 2007.

J. Y. Kim, S. H. Cho, H. N. Joo, M. S. R. Cho, M. Tsuji, I. J. Park, G. T. Chung, J. W. Ju, H. I. Cheun, H. W. Lee, Y. H. Lee and T. S. Kim. “First case of human Babesiosis in Korea: Detection and characterization of a novel type of Babesia sp. (KO1) similar to ovine Babesia”. Journal of Clinical Microbiology, vol. 45, pp. 2084- 2087, 2015.

S. Naz, A. Maqbool, S. Ahmed, K. Ashraf, N. Ahmed, K. Saeed, M. Latif, J. Iqbal, Z. Ali, K. Shafi and I. A. Nagra. “Prevalence of theileriosis in small ruminants Lahore-Pakistan”. Journal of Veterinary and Animal Science, vol. 2, pp. 16-20, 2012.

K. Altay, M. Aktas and N. Dumanli. “Detection of Babesia ovis by PCR in Rhipicephalus bursa collected from naturally infested sheep and goats”. Research in Veterinary Science, vol. 85, pp. 116-119, 2007.

A. Cakmack, A. Inci and Z. Kararer. “Seroprevalence of Babesia ovis in sheep and goats on Cankiri region”. Acta Parasitologica Turcica, vol. 22, pp. 73-76, 2020.

E. J. L. Soulsby. “Helminth, Arthropoda and Protozoa of Domesticated Animals”. Vol. 14. Bailler Tindall, London, 1982, pp. 456-471.

B. Fivaz, T. Petney and I. Horak. “Tick Vector Biology Medicine and Veterinary Aspects”. Vol. 45. Springer-Verlag, Berlin Heidelberg, 2020, p. 28.

B. A. Allsopp, H. A. Baylis, M. T. Allsopp, T. Cavalier-Smith, R. P. Bishop, D. M. Carrington, B. Sohanpal and P. Spooner. “Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences”. Parasitology, vol. 107, pp. 157-165, 1993.

S. Durrani, Z. Khan, R. M. Khattak, M. Andleeb, M. Ali, H. Hameed, A. Taqddas, M. Faryal, S. Kiran, M. Riaz, R. S. Shiek, M. Ali, F. Iqbal and M. Andleeb. “A comparison of the presence of Theileria ovis by PCR amplification of their SSU rRNA gene in small ruminants from two provinces of Pakistan”. Asian Pacific Journal of Tropical Disease, vol. 2, pp. 43-47, 2012.

A. Inci, A. Ica, A. Yildirim and O. Duzlu. “Identification of Babesia and Theileria species in small ruminants in Central Anatolia (Turkey) via reverse line blotting”. Turkish Journal of Veterinary and Animal Sciences, vol. 34, pp. 205-210, 2010.

K. T. Freiedhoff. “Tick-borne disease of sheep and goats caused by Babesia, Theileria or Anaplasma spp”. Parassitologia, vol. 39, pp. 99-109, 1997.

D. Nagore, J. García-Sanmartín, A. L. García-Pírez and R. A. Juste and A. Hurtado. “Identification, genetic diversity and prevalence of Theileria and Babesia species in a sheep population from Northern Spain”. International Journal for Parasitology, vol. 34, pp. 1059- 1067, 2004.

A. Rafiai. “Veterinary and comparative entomology”. Current Medicinal Chemistry, vol. 19, pp. 1504-1518, 2012.

J. Mosqueda, A. Olvera-Ramirez, G. Aguilar-Tipacamu and G. J. Canto. “Current advances in detection and treatment of Babesiosis”. Current Medicinal Chemistry, vol. 19, pp. 1504-1518, 2012.

F. Iqbal, M. Ali, M. Fatima, S. Shahnawaz, S. Zulifqar, R. Fatima, R. S. Shaikh, A. S. Shaikh, M. Aktas and M. Ali. “A study on prevalence and determination of the risk factors of infection with Babesia ovis in small ruminants from Southern Punjab (Pakistan) by PCR amplification”. Parasite, vol. 18, pp. 229-234, 2011.

L. William, N. Nicholson, N. Richard and M. Brown. In: “Medical and Veterinary Entomology”. 3rd ed. Georgia Southern University, Statesboro, GA, United States, 2019, pp. 51-65.

A. M. Zajac and G. A. Conboy. “Veterinary Clinical Parasitology”. Vol. 7. Blackwell Publishing Ltd., UK, 2000, pp. 172-175.

S. Kage, G. S. Mamatha, J. N. Lakkundi and B. P. Shivashankar. “Detection of incidence of Babesia spp. in sheep and goats by parasitological diagnostic techniques”. Journal of Parasitic Diseases, vol. 43, pp. 452-457, 2019.

Z. S. Dehkordi, S. Zakeri, S. Nabian, A. Bahonar, F. Ghasemi and F. Noorollahi. “Molecular and biomorphometrical identification of ovine Babesiosis in Iran”. Iranian Journal of Parasitology, vol. 5, pp. 21-30, 2010.

M. Tousli and S. Rahbari. “Investigation of seroepidemiology in sheep in different regions of Iran”. Veterinary Journal, vol. 53, pp. 57-65, 1998.

B. Papadopoulos, N. M. Perie and G. Uilenberg. “Piroplasms of domestic animals in the Macdonia region of Greece. 1. Serological cross-reactions”. Veterinary Parasitology, vol. 63, pp. 41-56, 1995.

A. Clmak, S. Dincer and Z. Karer. “Studies on the serological diagnosis of Babesia ovis infection in Samsun area”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 38, pp. 242-251, 2018.

Published

2023-08-08

How to Cite

Hossein, M. A. (2023). Identification of Blood Protozoa Infestation Transmitted by Vector Tikes among Awassi Sheep Herds in Kifri City, Kurdistan Region of Iraq. UHD Journal of Science and Technology, 7(2), 1–5. https://doi.org/10.21928/uhdjst.v7n2y2023.pp1-5

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Articles