Suspected Protective Effect of Eruca Sativa Leaves Extract on Sperm Abnormalities in Male Albino Mice Treated with Hypericum triqutrifolium aqueous crude extract

Authors

  • Chro Ghafoor Raouf Department of Biology, College of Science, University of Sulaimani, Sulaimani, Kurdistan, Iraq
  • Mahmood Othman Ahmad Department of Biology, College of Science, University of Sulaimani, Sulaimani, Kurdistan, Iraq

DOI:

https://doi.org/10.21928/uhdjst.v3n2y2019.pp87-92

Keywords:

Eruca sativa, Hypericum triquetrifolium, Sperm morphology

Abstract

 

 This study was carried out to evaluate the effect of Eruca sativa on the cytotoxic effect of Hypericum triquetrifolium on sperm abnormalities in albino mice. Leaves of E. sativa and aerial parts of H. triquetrifolium were dried in shade and grinded and their aqueous extracts were used for the treatments to study their effect on sperm morphology. Treated groups were injected with a single dose of 38 mg/kg body weight (BW) of Hypericum subcutaneously, while the Eruca groups were orally administered with 250 mg/kg BW twice/week for 2 weeks. After the exposure of H. triquetrifolium, the frequency of abnormal sperms showed a highly significant induction of sperm abnormalities; separated head from tail sperms, swollen head, hookless, defective head, and hook. However, the Eruca group showed no obvious abnormalities in sperm morphology, while in cotreatment with both Eruca and Hypericum (H/E) groups, there was an extremely significant decrease (P < 0.0001) in the abnormal sperms. In conclusion, it appeared that E. sativa could prevent or at least minimize the damages that Hypericum toxins would make on the sperm morphology significantly.

References

54, pp. 67-78, 1988.
[6] G. Mradu, S. Saumyakanti, M. Sohini and M. Arup. “HPLC profiles of standard phenolic compounds present in medicinal plants”. International Journal of Pharmacognosy and Phytochemical Research, vol. 4, pp. 162-167, 2012.
[7] S. Alqasoumi, M. Al-Sohaibani, T. Al-Howiriny, M. Al-Yahya and S. Rafatullah. “Rocket “Eruca sativa”: A salad herb with potential gastric anti-ulcer activity.” World Journal of Gastroenterology, vol. 15, pp. 1958-1965, 2009.
[8] M. I. A. Al-Farwachii. “ARAN (Hypericum crispum) Poisoning and its Effect on the Phagocytosis and Antibodies in Rabbits.” M.Sc. Thesis, University of Mosul (Arabic), 1997.
[9] M. D. Kako, I. I. Al-Sultanand and A. N. Saleem. “Studies of sheep experimentally poisoned with Hypericum perforatum.” Veterinary and Human Toxicology, vol. 35, pp. 298-300, 1993.
[10] A. Pandey and S. Tripathi. “Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug.” Journal of Pharmacognosy and Phytochemistry, vol. 2, no. 5, pp. 115-119, 2014.
[11] A. K. Sharma and A. Sharma. “Chromosome Techniques”. 3rd ed. Butter Worth, London, p. 486, 1980.
[12] M. N. Ansari, M. A. Ganaie, T. H. Khan and G. A. Soliman. “Protective role of Eruca sativa extract against testicular damage in streptozotocin-diabetic rats.” International Journal of Biology, Pharmacy and Allied Sciences, vol. 3, no. 7, pp. 1067-1083, 2014.
[13] E. F. Oakberg. “Differential spermatogonial stem-cell survival and mutation frequency.” Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 50, no. 3, pp. 327-340, 1978.
[14] D. M. De Kretser, K. L. Loveland, A. Meinhardt, D. Simorangkir and N. Wreford. “Spermatogenesis.” Human Reproduction, vol. 13 Suppl 1, pp. 1-8, 1998.
[15] M. Emtenan, E. M. Hanafi, R. M. Hegazy and H. A. Riad. “Bio-protective effect of Eruca sativa seed oil against the hazardous effect of aflatoxin b1 in male rabbits.” International Journal of Academic Research, vol. 2, pp. 67-74, 2010.
[16] M. Koubaa, D. Driss, F. Bouaziz, R. E. Ghorbel and S. E. Chaabouni. “Antioxidant and antimicrobial activities of solven extract obtained from rocket (Eruca sativa L.) flowers.” Free Radicals and Antioxidants, vol. 5, no. 1, pp. 29-34, 2015.
[17] P. J. Chenoweth. “Genetic sperm defects.” Theriogenology, vol. 64, no. 3, pp. 457-468, 2005.
[18] R. R. Ondrizek, P. J. Chan, W. C. Patton and A. King. “An alternative medicine study of herbal effects on the penetration of zona-free hamster oocytes and the integrity of sperm deoxyribonucleic acid.” Fertility and Sterility, vol. 71, no. 3, pp. 517-522, 1999.
[19] B. M. Mohammed and S. K. Kheravii. “Evaluation of genotoxic potential of Hypericum triquetrifolium extract in somatic and germ cells of male albino mice.” Research Opinions in Animal and Veterinary Sciences, vol. 1, no. 4, 231-239, 2011.
[20] J. Barillari, D. Canistro, M. Paolini, F. Ferroni, G. F. Pedulli, R. Iori and L. Valgimigli. “Direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (Eruca sativa Mill.) seeds and sprouts.” Journal of Agricultural and Food Chemistry, vol. 53, no. 7, pp. 2475-2482, 2005.
[21] B. M. A. Mohammed and J. A. H. Ali. “Synergistic effects of bleomycin and Hypericum triquetrifolium extract on bone marrow and germ cells of albino mice.” Journal of Global Pharma Technology, vol. 10, no. 5, pp. 203-212, 2009.
[22] B. N. Al-Okaily and Z. M. Al-Shammari. “The impact of Eruca sativa seeds on Leydig’s cells number and hormonal profile in cadmium exposed rats.” Kufa Journal for Veterinary Medical Sciences, vol. 7, no. 2, pp. 241-253, 2016.
[23] J. Jin, O. A. Koroleva, T. Gibson, J. Swanston, J. Magan, Y. Zhang, I. R. Rowland and C. Wagstaff. “Analysis of phytochemical composition and chemoprotective capacity of rocket (Eruca sativa and Diplotaxis tenuifolia) leafy salad following cultivation in different environments.” Journal of Agricultural and Food Chemistry, vol. 57, no. 12, pp. 5227-5234, 2009.
[24] A. J. Nwofal. “The Role of Ethanolic Extract of Salad Rockets (Eruca sativa) Leaves on the Performance of Male Reproductive System in Oxidative Stressed Rats.” PhD Thesis/College of Veterinary Medicine University, Baghdad, 2014.
[25] A. R. S. Mona and A. M. Nehal. “Histological and quantitative study of the effect of Eruca sativa seed oil on the testis of albino rat.” The Egyptian Journal of Hospital Medicine, vol. 2, pp. 148-162, 2001.
[26] D. Ates and O. Erdogrul. “Antimicrobial activates of various medicinal and commercial plant extracts.” Turkish Journal of Biology, vol. 27, pp. 157-162, 2003.
[27] Z. F. Hussein. “Study the effect of Eruca sativa leaves extract on male fertility in albino mice.” Al-Nahrain Journal of Science, vol. 6, no. 1, pp. 143-146, 2013.

Published

2019-11-14

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Articles