Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq
DOI:
https://doi.org/10.21928/uhdjst.v4n2y2020.pp117-122Keywords:
Hepatitis B Virus, Hepatitis B Surface Antigen, Enzyme-linked Immunosorbent Assay, Gold In-tube, Realtime Polymerase Chain ReactionAbstract
Hepatitis B virus infection is caused by the hepatitis B virus, a major global health problem. This infection can lead to chronic conditions, followed by cirrhosis and hepatocellular carcinoma (HCC). The current study was aimed to detect HBV using serological and molecular techniques. During 2019, 300 blood samples were collected from Kurdistan Center for Hepatology and Gastroenterology in Sulaimani city. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) techniques were used for the detection of HBsAg and HBV DNA, respectively. Obtained results were revealed that 92 out of 300 tested patients (30.66%) seropositive for HBsAg. Among 92 seropositive patients, 53 were shown positive results for HBV DNA by RT-PCR. Dental clinic visiting and dialysis were among the important risk factors for HBV transmission. The vast majority of positive results were among males. Smokers showed relatively high rates of positive results. One-third of the referred patients who had liver complaints were positive for HBsAg. More than half of the seropositive patients showed RT-PCR positive results. It was concluded that the molecular method (RT-PCR) is more sensitive and gives a more accurate result than serology (ELISA). Therefore, it can be used as a diagnostic tool for HBV detection.
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[15] S. A. Hama. “Hepatitis C seropositivity and RNA detection among Type2 diabetic patients in sulaimani governorate-Iraqi Kurdistan Region”. Journal of Zankoy Sulaimani A, vol. 18, no. 4, pp. 1-8, 2016.
[16] Z. Nokhodian, M. R. Yazdani, M. Yaran, P. Shoaei, M. Mirian, B. Ataei, A. Babak, M. Ataie. “Prevalence and risk factors of HIV, syphilis, hepatitis B and C among female prisoners in Isfahan, Iran”. Hepatitis Monthly, vol. 12, pp. 442-447, 2012.
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[24] J. Paoli, A. C. Wortmann, M. G. Klein, V. R. Z. Pereira, A. M. Cirolini, B. A. de Godoy, N. J. R. Fagundes, J. M. Wolf, V. R. Lunge and D. Simon. “HBV epidemiology and genetic diversity in an area of high prevalence of hepatitis B in Southern Brazil”. The Brazilian Journal of Infectious Diseases, vol. 22, no. 4, pp. 294-304, 2018.
[25] P. F. Belaunzaran-Zamudio, J. L. Mosqueda-Gomez, A. Macias- Hernandez, S. Rodríguez-Ramírez, J. Sierra-Madero and C. Beyrer. Burden of HIV, syphilis, and hepatitis B and C among inmates in a prison state system in Mexico. AIDS Research and Human Retroviruses, vol. 33, no. 6, pp. 524-533, 2017.
[26] M. A. A. Al Kasem, M. Al-Kebsi Abbas, M. M. Ebtihal and A. A. S. Hassan. “Hepatitis B virus among dental clinic workers and the risk factors contributing for its infection”. Journal of Dentistry and Oral Health, vol. 1, no. 2, ???, 2018.
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[28] M. Gencay, A. Seffner, S. Pabinger, J. Gautier, P. Gohl, M. Weizenegger, D. Neofytos, R. Batrla, A. Woeste, H. S. Kim, G. Westergaard, C. Reinsch, E. Brill, P. T. T. Thuy, B. H. Hoang, M. Sonderup, C. W. Spearman, G. Brancaccio, M. Fasano, G. B. Gaeta, T. Santantonio and W. E. Kaminski. “Detection of in vivo hepatitis B virus surface antigen mutations a comparison of four routine screening assays”. Journal of Viral Hepatitis, vol. 25, no. 10, pp. 1132-1138, 2018.
[29] H. J. Lee, S. Y. Kim, S. M. Lee, J. Heo, H. H. Kim and C. L. Chang. “Elecsys hepatitis B surface antigen quantitative assay: Performance evaluation and correlation with hepatitis B virus DNA during 96 weeks of follow-up in chronic hepatitis B patients”. Annals of Laboratory Medicine, vol. 32, no. 6, pp. 420-425, 2012.
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Published
2020-11-26
How to Cite
Abdulla, R. S., & Hama, S. A. (2020). Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq. UHD Journal of Science and Technology, 4(2), 117–122. https://doi.org/10.21928/uhdjst.v4n2y2020.pp117-122
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